sirt1 (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc sirt1
Sirt1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 211 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phospho+enos+thr495/Phospho-eNOS+(Thr495)+Antibody/pm41603485-123-30-31
Average 95 stars, based on 211 article reviews

sirt1 - by Bioz Stars, 2026-09

95/100 stars

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Membrane:

Article Title: Endothelial dysfunction and transcriptome aberration in mouse aortas induced by black phosphorus quantum dots and nanosheets.
Article Snippet: Black phosphorus (BP) nanomaterials have shown great potential in versatile applications including biomedicine and potentially interact with vessel walls following intravenous injection in biomedical usage or environmental exposure.. However, it remains unknown whether the exposure to BP nanomaterials induces alterations of the endothelium and further vascular injury.. Herein, the endothelial function of human umbilical vein endothelial cells (HUVECs) and the structure and transcriptome of C57BL/6 mouse aortas are evaluated after the exposure to BP quantum dots (BPQDs) and nanosheets (BPNSs).

Incubation:

Data-independent acquisition:

Article Title: AQP1 differentially orchestrates endothelial cell senescence
Article Snippet: .. Antibodies against phospho- AMPKα Thr172 (1:1000; 2535), total-AMPKα (1: 1000; 2532), phospho-eNOS Ser1177 (1:1000; 9517), phospho-eNOS Thr495 (1:1000; 9574), total-eNOS (1: 1000; 32,027), Histone H3 (1:1000; 9715), Mef2a (1:1000; 9736), and phospho-Histone H2A.X Ser 139 (1:100 and 1:1000; 80,312) were purchased from Cell Signaling Technology; antibodies against VCAM-1 (1:100, 1:200 and 1:1000; MA5-31965), and VE Cadherin (1:100; PA5-19612) were obtained from Invitrogen; antibody against AQP1 (1:750 and 1:1000; ab168387), phospho-HDAC4 Ser632 (1:250 and 1:1000; ab39408), and total-HDAC4 (1:1000; ab12172) were obtained from abcam; antibody against CD31 (1:50; DIA-310) was purchased from Dianova; antibody against H3ac (Pan-Acetyl) (1:500; sc-518,011) was obtained from Santa Cruz Biotechnology; antibody against p16 INK4A (1:100; ZRB1437) was purchased from Merck Millipore; antibodies against goat anti-rabbit IgG-HRP (1:2000; 4030–05) and goat anti-mouse IgG-HRP (1:2000; 1036–05) were purchased from Southern Biotechnology. .. Proteins were extracted from in ice-cold RIPA lysis buffer (ThermoFisher Scientific) or nuclear and cytoplasmic extraction reagents (for protein isolation from nuclear and cytosolic fractions) supplemented with protease and phosphatase inhibitors cocktails (ThermoFisher Scientific).

Article Title: AQP1 Differentially Orchestrates Endothelial Cell Senescence
Article Snippet: .. Antibodies against phospho-AMPKα Thr172 (1:1000; 2535), total-AMPKα (1: 1000; 2532), phospho-eNOS Ser1177 (1:1000; 9517), phospho-eNOS Thr495 (1:1000; 9574), total-eNOS (1: 1000; 32027), Histone H3 (1:1000; 9715), Mef2a (1:1000; 9736), and phospho-Histone H2A.X Ser139 (1:100 and 1:1000; 80312) were purchased from Cell Signaling Technology; antibodies against VCAM-1 (1:100, 1:200 and 1:1000; MA5-31965), and VE Cadherin (1:100; PA5-19612) were obtained from Invitrogen; antibody against AQP1 (1:750 and 1:1000; ab168387), phospho-HDAC4 Ser632 (1:250 and 1:1000; ab39408), and total-HDAC4 (1:1000; ab12172) were obtained from abcam; antibody against CD31 (1:50; DIA-310) was purchased from Dianova; antibody against H3ac (Pan-Acetyl) (1:500; sc-518011) was obtained from Santa Cruz Biotechnology; antibody against p16 INK4A (1:100; ZRB1437) was purchased from Merck Millipore; antibodies against goat anti-rabbit IgG-HRP (1:2000; 4030-05) and goat anti-mouse IgG-HRP (1:2000; 1036-05) were purchased from Southern Biotechnology. .. Proteins were extracted from in ice-cold RIPA lysis buffer (ThermoFisher Scientific) or nuclear and cytoplasmic extraction reagents (for protein isolation from nuclear and cytosolic fractions) supplemented with protease and phosphatase inhibitors cocktails (ThermoFisher Scientific).

Article Title: AQP1 differentially orchestrates endothelial cell senescence.
Article Snippet: .. Antibodies against phospho- AMPKα Thr172 (1:1000; 2535), totalAMPKα (1: 1000; 2532), phospho-eNOS Ser1177 (1:1000; 9517), phospho-eNOS Thr495 (1:1000; 9574), total-eNOS (1: 1000; 32,027), Histone H3 (1:1000; 9715), Mef2a (1:1000; 9736), and phospho-Histone H2A.X Ser 139 (1:100 and 1:1000; 80,312) were purchased from Cell Signaling Technology; antibodies against VCAM-1 (1:100, 1:200 and 1:1000; MA5-31965), and VE Cadherin (1:100; PA5-19612) were obtained from Invitrogen; antibody against AQP1 (1:750 and 1:1000; ab168387), phospho-HDAC4 Ser632 (1:250 and 1:1000; ab39408), and total-HDAC4 (1:1000; ab12172) were obtained from abcam; antibody against CD31 (1:50; DIA-310) was purchased from Dianova; antibody against H3ac (Pan-Acetyl) (1:500; sc-518,011) was obtained from Santa Cruz Biotechnology; antibody against p16INK4A (1:100; ZRB1437) was purchased from Merck Millipore; antibodies against goat anti-rabbit IgG-HRP (1:2000; 4030–05) and goat anti-mouse IgG-HRP (1:2000; 1036–05) were purchased from Southern Biotechnology. .. Proteins were extracted from in ice-cold RIPA lysis buffer (ThermoFisher Scientific) or nuclear and cytoplasmic extraction reagents (for protein isolation from nuclear and cytosolic fractions) supplemented with protease and phosphatase inhibitors cocktails (ThermoFisher Scientific).

other:

Article Title: Gut microbiota-dependent increase in phenylacetic acid induces endothelial cell senescence during aging
Article Snippet: Antibodies against phospho-CaMKII Thr286 (1:1000; 12716), total-CaMKII (1: 1000; 4436), phospho-HDAC4 Ser632 (1:1000; 3424), total-HDAC4 (1:1000; 7628), H3 (1:1000; 9715), phospho-eNOS Ser1177 (1:1000; 9517), phospho-eNOS Thr495 (1:1000; 9574), total-eNOS (1: 1000; 32027), and phospho-Histone H2A.X Ser139 (1:1000; 80312) were purchased from Cell Signaling Technology; antibodies against VCAM-1 (1:1000; MA5-31965), IL6 (1:1000; M-620), NADPH oxidase 2 (NOX2) (1:1000; PA5-76034), and VE Cadherin (1:100; PA5-19612) were obtained from Invitrogen; antibody against CD31 (1:100; 14-0311-82) was purchased from eBioscience; antibody against Histone H3ac (Pan-Acetyl) (1:500; sc-518011) was obtained from Santa Cruz Biotechnology; antibody against p16 INK4A (1:100; ZRB1437) was purchased from Merck Millipore; antibody against phospho-HDAC4 Ser632 (1:100; ab39408-1001) and conjugated secondary antibodies against Alexa Fluor ® 488 (1:2000; ab150157) and Alexa Fluor ® 647 (1:2000; ab150075) were obtained from abcam; antibodies against goat anti-rabbit IgG-HRP (1:2000; 4030-05) and goat anti-mouse IgG-HRP (1:2000; 1036-05) were purchased from Southern Biotechnology.

Western Blot:

Article Title: Distinct Signaling Functions of Rap1 Isoforms in NO Release From Endothelium
Article Snippet: .. The following antibodies were used for Western Blot analysis: antibodies against phospho-eNOS (Ser-1177) (BD Biosciences #612392), phospho-eNOS (Thr495) (Cell Signaling Technologies #9574), total eNOS (Cell Signaling Technologies #32027), Rap1A/Rap1B (Cell Signaling Technologies #2399), and β-actin (Santa Cruz Biotechnology #sc-47778). .. Rap1 rabbit monoclonal antibody (clone 26B4, Cell Signaling Technologies #2399) was used for GTP-Rap1 pull-down assay.

Article Title: Hepatic phosphate uptake and subsequent nerve-mediated phosphaturia are crucial for phosphate homeostasis following portal vein passage of phosphate in rats
Article Snippet: .. Antibodies against specific molecules were obtained as follows: CD68 (catalog: ab25212, Abcam Cambridge, UK), hepatic sinusoid endothelial cells (SE-1) (catalog: NB110-68095, Novusbio, Colorado, USA), Alexa Fluor 555 donkey anti-rabbit IgG antibody (catalog: A-31572, Invitrogen), endothelial nitric oxide synthase (eNOS) (catalog: #9572, Cell Signaling, Danvers, USA), phospho-eNOS Thr495 (catalog: #9574, Cell Signaling), and horseradish peroxidase (HRP)-conjugated 2 nd Ab for WB (catalog: P0448, DAKO, Santa Clara, USA). ..

Review